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Comment 09

Please cite this contribution as follows: Ilya [Bozo]. Alexey, I’ll answer in order: 1. You said: “What. Blog comment, Maximow Award contest, May 2012. Cell Ther Transplant/Maximow Award, May 2012;blog-comment_09. doi:10.3205/maximowaward_2012_blog-comment_09

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Ilya. May 31, 2012 at 7:59 am

Alexey,

I’ll answer in order:
1. You said: “What do you mean under “clonal analysis”? What assay? Lineage tracing? Colony assay? What classical assay for HSC self-renewal are you talking about? Can your give a reference?”
I have meant a “colony assay”. I’m not going to go far for the references. For example, it is the Morita’s acticle (2010) mentioned by you earlier.

2. You said: “As I told you, in primary, you transplant a single cell”.
First, theoretically, the method implies the transplantation from one to ten or more cells. Secondly, in practice it is more than one cell always. Explain to me how can get the expansion of HSCs after transplantation of a single cell? The most HSCs does not reach into stroma and “die” even when it is a therapeutic transplantation in clinical practice (these are the words of professor Afanasiev, V Annual International Symposium “Actual issues of gene and cellular technologies”).

3. You said: “Please give an example, how “clonal analysis” would assess HSC self-renewal better than transplantation assays”.
Clonal analysis allows us to manipulate a single cell, but serial transplantation does not allow to do it.

4. I do not exclude that the population of HSCs ever can be divided into subpopulations. However, the criteria for separating on subpopulations must be very substantial and have a practical sense. Stable functional differences or identified differences of origin can be considered as such criteria. If we see that in some conditions cells have some characteristics and these same cells have other characteristics in other conditions, it is absolutely normal. This is not a reason to divided its into subpopulations.

You said: “We use a special mice – genetically identical…” Is it the clones? if it is not, then there is no genetic identity too.

Thanks for an interesting discussion.

This contribution is provided under the following license: Creative Commons Attribution 3.0 Unported (CC BY 3.0)

Please cite this contribution as follows: Ilya [Bozo]. Alexey, I’ll answer in order: 1. You said: “What. Blog comment, Maximow Award contest, May 2012. Cell Ther Transplant/Maximow Award, May 2012;blog-comment_09. doi:10.3205/maximowaward_2012_blog-comment_09

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